Journal: Scientific Reports
Article Title: Elevated CO 2 regulates the Wnt signaling pathway in mammals, Drosophila melanogaster and Caenorhabditis elegans
doi: 10.1038/s41598-019-54683-0
Figure Lengend Snippet: Validation of mouse multi-tissue microarray analysis. ( a,b) Validation in mouse tissues. Fzd9 , Wnt4 , Wnt7a , and Wnt8b expression in the lung (n = 6–7) ( a ) and diaphragm skeletal muscle (n = 4–5) ( b ) from mice exposed to normoxic hypercapnia for 7 days. NC, normocapnia; HC, hypercapnia. ( c,d) Validation in mouse lung and skeletal muscle cells. Fzd9 and Wnt7a expressions in MLE-12 cells (Ctrl, n = 22–23; 20%CO 2 , n = 5 per group), ASM cells (Ctrl, n = 22–23; 20%CO 2 , n = 5 per group) ( c ), or C2C12 myoblast (Ctrl, n = 18–19; 20%CO 2 , n = 4–5 per group) or myotube (Ctrl, n = 14–15; 20%CO 2 , n = 3–4 per group) ( d ) exposed to high CO 2 for up to 24 hours (c) or 6 hours (d). Ctrl, control conditions. All values are represented as mean with error bars shown as the 95% confidence interval. *p < 0.05, **p < 0.01, ***p < 0.001, unpaired two-tailed Student’s t test or one-way ANOVA with Dunnett’s post hoc test.
Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO 2 carbon dioxide controller (BioSpherix).
Techniques: Microarray, Expressing, Control, Two Tailed Test